Skip to content Skip to navigation

Gold Nanoparticle Based FRET Asssay for Detection of DNA Cleavage

Paresh Chandra Ray, Angela Fortner and Gopala K. Darbha
Department of Chemistry, Jackson State University, Jackson, MS, USA
Acknowledgements: Dr. Ray thanks NSF-CRIFMU grant # 0443547, NSF-PREM grant # DMR-0611539, and NIH-RCMI grant # G12RR13459 for their generous funding. A.F. thanks NSF/LSMAMP grant # HRD-0115807 for her scholarship during matriculation.

This represents the first nanoparticle-based fluorescence assay for probing single-strand DNA nuclease activity. Our simple assay with extremely high sensitivity will make it widely applicable and highly useful for convenient characterization of DNA/RNA cleavage reactions Given the simplicity, speed, and sensitivity of this approach, the described methodology could easily be extended to a high throughput format and become a new method of choice in HIV proteases and matrix metalloproteases (MMP) as well as the proteins involved in cellular apoptosis

(Published in J. Phys. Chem. B, as a letter, J. Phys. Chem. B, 2006, 110, 20745).